The Integrin alpha2 subunit, also known as CD49b and VLA-2 alpha subunit, forms a heterodimer with the Integrin Beta1 (CD29) or Beta4 (CD104) subunits and binds laminin.
Applications:
Suitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
DirectELISA: 0.5-1.0ug/ml detection limit for rhITGA2 is ~ 2ng/well.
Western Blot: 1-2ug/ml detection limit for rhITGA2 is ~ 5ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.