The a3 subunit, also known as CD49c and VLA-3 a subunit, forms a non-covalent heterodimer with the Integrin b1 subunit (CD29). Integrin a3b1 is a receptor for laminin, fibronectin, collagen, epiligrin, and thrombospondin.
Hybridoma:
Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a human milk epithelial cell line.
Endotoxin Level:
< 0.1 EU per 1ug of the antibody as determined by the LAL method.
Applications:
Immunohistochemistry, Flow Cytometry
Recommended Dilution:
Immunohistochemistry: This antibody was used to detect Integrin a3 subunit in paraformaldehyde-fixed HT1080 fibroscarcoma cells. The recommended working dilution is 25ug/mL. For chromogenic detection of labeling, the use of Cell and Tissue Staining Kits is recommended.
Flow Cytometry: Dilute this antibody to 25ug/mL and add 10ul of this solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10ul of a 25ug/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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