Home  >  Products  >  CD49d (CD49 antigen-like family member D, CD49d, CD49D, IA4, Integrin alpha-4, Integrin alpha-IV, MGC90518, VLA-4 subunit alpha)
CD49d (CD49 antigen-like family member D, CD49d, CD49D, IA4, Integrin alpha-4, Integrin alpha-IV, MGC90518, VLA-4 subunit alpha)

CD49d (CD49 antigen-like family member D, CD49d, CD49D, IA4, Integrin alpha-4, Integrin alpha-IV, MGC90518, VLA-4 subunit alpha)

Cat no: C2404-42B

Supplier: United States Biological
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Human CD49d molecules are integrin alpha 4 chains that are expressed as heterodimers with CD29 (beta1 integrin) or (beta 7 integrin) and function as adhesion receptors. CD49d/CD29 is mainly expressed on thymocytes and B cells with increased expression on activated T cells. The ligands for CD49d/CD29 include VCAM-1 and fibronectin. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: Fixed cells or tissues Flow Cytometry: 5x10e5 cells were washed incubated 45 min on ice with 80ul of anti-CD49d antibody diluted to 5ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.52 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2404-42B
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry
Size: 100ug
Form: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1617-1618, 2. T. Sato, (1995) J Immunol 155:2938-2947.
Additional info: Recognizes human CD49d.

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