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CD49d (Integrin alpha4)

Cat no: C2404-71D


Supplier: United States Biological
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Integrins are a/b heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migra- tion, as well as in growth and survival (1,2). The integrin family contains at least 18 a and 8 b subunits that form 24 known integrins with distinct tissue distribution and over- lapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2404-71D
Reactivities: Human
Hosts: Rabbit
Applications: Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100ug/ml BSA and 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by peptide affinity chromatography.
References: 1. Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2:91
Additional info: Detects endogenous levels of human integrin a4 mature protein (150kDa), a4 precursor protein (140kDa), as well as 70 kDa cleaved C-terminal alpha 4 fragment.

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