Home  >  Products  >  CD49f (CD49 Antigen-like Family Member F, CD49f Antigen, Integrin alpha 6, Integrin alpha-6, ITGA6, ITGA6B, VLA-6) (Low Endotoxin)
CD49f (CD49 Antigen-like Family Member F, CD49f Antigen, Integrin alpha 6, Integrin alpha-6, ITGA6, ITGA6B, VLA-6) (Low Endotoxin)

CD49f (CD49 Antigen-like Family Member F, CD49f Antigen, Integrin alpha 6, Integrin alpha-6, ITGA6, ITGA6B, VLA-6) (Low Endotoxin)

Cat no: C2585-25D

Supplier: United States Biological
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CD49F (VLA-6 alpha chain), a 120kD cell surface glycoprotein that can form distinct complexes with CD29 (VLA beta-chain), resulting in the VLA-6 (alpha-6 beta-1) complex, which is expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6 beta-4 complex which is expressed on various human epithelial cells. Clone NKI-GoH3 reacts with platelets, megakaryocytes, T lymphocytes and common Acute Lymphoblastic Leukaemia cells (alpha-6 beta-1). In immunohistology the monoclonal antibody reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytotrophoblasts.VLA-6 is an important mediator of cell binding to laminin. Studies suggest that clone GoH3 specifically blocks the binding of cells to the E8 fragment of laminin (7). Applications: Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and functional applications. Other applications have not been tested. Recommended Dilutions: Flow cytometry: 1:25-1:50 Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Immunohistochemistry Positive control: Human Tonsil Endotoxin: (same/less than)0.01EU/ug Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2585-25D
Reactivities: Human, Mouse, Non-Human Primate, Porcine, Sheep
Hosts: Rat
Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Size: 500ug
Form: Supplied as a liquid in PBS, pH 7.4. No preservatives added.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein G affinity chromatography. Endotoxin: <0.01EU/ug
Alternative names: DKFZp686J01244, FLJ18737
References: 1. Jensen, K.B. et al. (2010) Assaying proliferation and differentiation capacity of stem cells using disaggregated adult mouse epidermis. 2010; Epub 2010 Apr 22. PubMed PMID: Nat Protoc. 5: 898-911.2. Soligo, D. et al. (1989) Immunohistochemical reacitivy on bone marrow and tissues of anti-VLA antibodies in the platelet panel, in Leucocyte Typing IV: White Cell Differentiation Antigens. Edited by Knapp, W. et al. Oxford University Press p1029-1032.3. Sonnenberg, A. et al. (1986) Development of mouse mammary gland: identification of stages in differentiation of luminal and myoepithelial cells using monoclonal antibodies and polyvalent antiserum against keratin. Histochem. Cytochem. 34: 1037-1046.4. Sonnenberg, A. et al . (1987) A complex of platelet glycoproteins Ic and IIa identified by a rat monoclonal antibody J. Biol. Chem. 262: 10376-10383.5. Helmer, M. et al. (1988) Multiple very late antigen (VLA) heterodimers on platelets. Evidence for distinct VLA-2, VLA-5 (fibronectin receptor), and VLA-6 structures J. Biol. Chem. 263: 7660-7665.6. Galkowska, H. et al. (1996) Reactivity of antibodies directed against human antigens with surface markers on canine leukocytes. Vet. Immunol. Immunopathol. 53: 329 - 334.7. Sonnenberg, A. et al. (1988) Laminin receptor on platelets is the integrin VLA-6. Nature. 336: 487 - 489.8. Sonnenberg, A. et al. (1990) Integrin recognition of different cell-binding fragments of laminin (P1, E3, E8) and evidence that alpha 6 beta 1 but not alpha 6 beta 4 functions as a major receptor for fragments E8. J. Cell. Biol. 110: 2145 - 2155.9. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp. Anim.49 (2): 97-11010. Sonneberg, A. et al. (1990) The alpha 6 beta 1 (VLA-6) and alpha 6 beta 4 protein complexes: tissue distribution and biochemical properties. J. Cell Sci. 96: 207 - 217.11. Sonnenberg, A., et al. (1988) Identification and characterization of a novel antigen complex on mouse mammary tumour cells using a monoclonal antibody against platelet glycoprotein Ic. J. Biol. Chem. 263: 14030 - 14038.12. Le Bellego, F. et al. (2005) Cytoskeleton reorganization mediates alpha6beta1 integrin-associated actions of laminin on proliferation and survival, but not on steroidogenesis of ovine granulosa cells. Reprod Biol Endocrinol. 3: 19.
Additional info: Recognizes human CD49F (VLA-6 alpha chain). Species crossreactivity: mouse, canine, porcine, Cynomolgus monkey, sheep.

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