Home  >  Products  >  CD49f (CD49 antigen-like family member F, CD49f, DKFZp686J01244, FLJ18737, Integrin alpha-6, ITGA6B, VLA-6)
CD49f (CD49 antigen-like family member F, CD49f, DKFZp686J01244, FLJ18737, Integrin alpha-6, ITGA6B, VLA-6)

CD49f (CD49 antigen-like family member F, CD49f, DKFZp686J01244, FLJ18737, Integrin alpha-6, ITGA6B, VLA-6)

Cat no: C2585-25E

Supplier: United States Biological
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Human CD49f molecules are integrin alpha-6 chains that are expressed as heterodimers with CD29 (beta1 integrin) or CD104 (beta4 integrin) and function as adhesion receptors. CD49f is expressed on platelets, monocytes, T lymphocytes and thymoctyes. The ligands for CD49f/CD29 include laminin and invasion. CD49f/CD29 has been shown to associate with CD63. Applications: Suitable for use in Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 cultured Raji human tumor cells were washed and incubated 45 min on ice with 80ml of anti-CD49f antibody at 10mg/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.78 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2585-25E
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. M. Liebert, et al, (1993) Hybridoma 12:67-80, 2. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1619-1620, 3. F. Berditchevski, et al, (1995) J Biol Chem 270:17784-17790.
Additional info: Recognizes human CD49f.

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