Home  >  Products  >  CD5 (CD5 Antigen, CD5 Molecule, LEU1, Lymphocyte Antigen CD5, Lymphocyte Antigen T1/Leu 1, Lymphocyte Glycoprotein T1/Leu1, p56 62, T cell Surface Glycoprotein CD5, T1)
CD5 (CD5 Antigen, CD5 Molecule, LEU1, Lymphocyte Antigen CD5, Lymphocyte Antigen T1/Leu 1, Lymphocyte Glycoprotein T1/Leu1, p56 62, T cell Surface Glycoprotein CD5, T1)

CD5 (CD5 Antigen, CD5 Molecule, LEU1, Lymphocyte Antigen CD5, Lymphocyte Antigen T1/Leu 1, Lymphocyte Glycoprotein T1/Leu1, p56 62, T cell Surface Glycoprotein CD5, T1)

Cat no: C2256-12K

Supplier: United States Biological
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The CD5 antigen is present on approximately 70% of normal peripheral blood lymphocytes and >90% of all T-lymphocytes. T-lymphocytes in the thymus stain weakly while peripheral T-cells stain strongly. Monocytes, granulocytes and NK cells are not stained (1). Anti-CD5 also reacts with a distinct subset normal B-lymphocytes, occasional cells in B-lymphocyte areas of spleen and lymph node, and most Ig+ B-chronic lymphoblastic leukemia (CLL) cells. The CD5 antigen has also been shown to be present on some lymphomas. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry (frozen and paraffin), and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:100; 10ul labels 10e6 cells in 100ul Immunohistochemistry (paraffin): PLP fixation is recommended. Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2256-12K
Reactivities: Rat
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Size: 100ug
Form: Supplied as a liquid in PBS, 0.09% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
References: 1. Dallman, M.J. et al. (1982) The roles of host and donor cells in the rejection of skin allografts by T cell-deprived rats injected with syngeneic T cells. Eur. J. Immunol. 12: 511-518, 2. Dallman, M.J. et al. (1984) MRC OX-19: A monoclonal antibody that labels rat T lymphocytes and augments in vitro proliferative responses. Eur. J. Immunol. 14: 260-267, 3. Huitinga, I. et al. (1995) Macrophages in T cell line-mediated, demyelinating, and chronic relapsing experimental autoimmune encephalomyelitis in Lewis rats. Clin. Exp. Immunol. 100: 344-351, 4. Whiteland, J.L. et al. (1995) Immunohistochemical Detection of T-Cell Subsets and other Leucocytes in Paraffin-embedded Rat and Mouse Tissues with Monoclonal Antibodies. J. Histochem. Cytochem. 43: 313-320.
Additional info: Recognizes rat CD5.

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