Home  >  Products  >  CD5 (CD5 Antigen, CD5 Molecule, LEU1, Lymphocyte Antigen CD5, Lymphocyte Antigen T1/Leu 1, Lymphocyte Glycoprotein T1/Leu1, p56 62, T cell Surface Glycoprotein CD5, T1) (FITC)
CD5 (CD5 Antigen, CD5 Molecule, LEU1, Lymphocyte Antigen CD5, Lymphocyte Antigen T1/Leu 1, Lymphocyte Glycoprotein T1/Leu1, p56 62, T cell Surface Glycoprotein CD5, T1) (FITC)

CD5 (CD5 Antigen, CD5 Molecule, LEU1, Lymphocyte Antigen CD5, Lymphocyte Antigen T1/Leu 1, Lymphocyte Glycoprotein T1/Leu1, p56 62, T cell Surface Glycoprotein CD5, T1) (FITC)

Cat no: C2256-12D3

Supplier: United States Biological
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Bovine CD5 is expressed by all mature T-lymphocytes and a subpopulation of B-lymphocytes. It is also expressed by mature medullary thymocytes and at a lower level by immature cortical thymocytes. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat-1:5; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Catalogue number: C2256-12D3
Reactivities: Bovine, Goat, Sheep
Hosts: Mouse
Applications: Flow Cytometry
Size: 100ug
Form: Supplied as a liquid, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
References: 1. Howard, C.J. et al. (1991) Individual antigens of cattle, bovine CD5 (BoCD5). Vet. Immunol. Immunopathol. 27: 55-60.2. Howard, C.J.et al. (1988) Two monoclonal antibodies (CC17 - CC29) recognizing an antigen (Bo5) on bovine T-lymphocytes, analagous to human CD5. Vet. Immunol. Immunopathol. 19: 127-139.3. Howard, C.J. et al. (1991) Competitive binding with putative Bo5 (CD5) cluster of monoclonal antibodies. Vet. Immunol. Immunopathol. 27: 147-151.4. Hein, W. R. et al. (1991) Comparison of reactivity of monoclonal antibodies on bovine, ovine and caprine tissues and on cells from other animal species. Vet. Immunol. Immunopathol. 27: 32-34.
Additional info: Recognizes bovine CD5. Reacts with BoCD5.1, which is a polymorphic antigen expressed on cells of all Bos Taurus animals and a small proportion of Bos Indicus animals. Species Crossreactivity: goat, sheep.

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