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CD5 (LEU1, Lymphocyte Antigen T1/Leu-1, T1, T-Cell Surface Glycoprotein CD5)

CD5 (LEU1, Lymphocyte Antigen T1/Leu-1, T1, T-Cell Surface Glycoprotein CD5)

Cat no: C2255-86E

Supplier: United States Biological
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Human CD5 is believed to be an alternative signaling molecule found on all mature T cells, on most thymocytes and on a subpopulation of mature B cells expressing autoantibodies. The ligand for CD5 is CD72. Recognizes epitope 2 of the CD5 molecule of approximately 67kD. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 cultured CEM human tumor cells were washed and incubated 45 min on ice with 80ul of anti-CD5 antibody at 5ug/ml. Cells were washed twice and incubated with 50ul of Goat anti-Mouse IgG/FITC at a 1:60 dilution factor, after which they were washed three times and fixed. Cells stained positive with a mean shift of 1.04 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2255-86E
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 100ug
Form: Supplied as a liquid in 50 mM sodium phosphate pH 7.5, 100 mM potassium chloride, 150mM sodium chloride. No preservative added.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. P.C.L. Beverly & R.E. Callard. "Protides of the Biological Fluids" (1981) Vol. 29:653-658, H. Peeters (ed.), Pergamon Press, Oxford. Leukocyte Typing IV (W. Knapp, et al, eds.) 2. Oxford University Press, Oxford (1989) p. 331-338. G.S. Wood & P.S. Freudenthal, (1992) Am J Pathol 141: 789-795. P.M. Lydyard, et al, (1993) Immunol Lett 38: 159-166.
Additional info: Recognizes epitope 2 of the human CD5 molecule of approximately 67 kd. Antibody UCHT2 does not stimulate CD5+ PBMNC.

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