CD5 (Lymphocyte antigen T1/Leu-1) is a transmembrane glycoprotein which has been implicated as a single-pass type I receptor in the regulation of T-cell proliferation (1). CD5 is part of the scavenger receptor cysteine-rich (SRCR) family group B and it is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is also expressed by T cell leukemia, lymphomas, activated T cells, and occasionally a subset of B cells. Mantle cell lymphomas (same as diffuse centrocytic lymphomas) are CD5 positive while the follicle center cell lymphomas are CD5 negative. A ligand for CD72/LYB-2, CD5 has been linked to negative regulation of T cell and B cell receptors signaling (2).
Applications:
Suitable for use in Immunocytochemistry, Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 1:2,000-1:10,000
Immunocytochemistry: 1:100
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.