CD5, Recombinant, Mouse, His-tag (Leu-1, Ly-1, T1)

CD5, also known as Leu-1, Ly-1, and T1, is a 67kD transmembrane glycoprotein in the scavenger receptor superfamily (1). Mature mouse CD5 consists of a 347 amino acid (aa) extracellular domain (ECD) with three scavenger receptor cysteine-rich (SRCR) domains, a 30 aa transmembrane segment, and a 93aa cytoplasmic domain (2). Within the ECD, mouse CD5 shares 55% and 82% aa sequence identity with human and rat CD5, respectively. The 52kD ECD can be cleaved from the cell surface and circulates in the serum (3). CD5 has been shown to interact homophilically, with CD72 on B cells, and with beta-glucan components of fungal cell walls (4-6). CD5 expression on developing thymocytes is positively regulated by signaling through the T cell antigen receptor (TCR) and is up-regulated on tolerized peripheral CD4+ cells (7, 8). It inhibits TCR signaling and promotes T cell nonresponsiveness and survival (8-10). CD5 signaling inhibits the generation of regulatory T cells but promotes the development of Th17 cells (11, 12). Within the B cell lineage, CD5 is expressed on B-1a cells, anergic B cells, and IL-10 producing regulatory B cells (13-16). Similarly to on T cells, it negatively regulates signaling through the B cell antigen receptor and supports peripheral B cell survival, anergy, and tolerance (13, 14, 16). In humans, B cells can produce an intracellularly-retained form of CD5 which lacks the signal peptide and a portion of the first SRCR domain (17). CD5 is also involved in the cellular entry of hepatitis C virus into T cells (18).\n\nSource:\nRecombinant protein corresponding to 24-370 from mouse CD5 fused to his-tag at C-terminal, NS0 derived.\n\nMolecular Weight: \n~39.2kD\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

145779

Size

50ug

Applications

ELISA

Form

Supplied as a lyophilized powder in MES, sodium chloride. Reconstitute in 100ug/ml in PBS.

Purity

~95% (SDS-PAGE)

References

1. Soldevila, G. et al. (2011) Curr. Opin. Immunol. 23: 310. 2. Huang, H.J. et al. (1987) Proc. Natl. Acad. Sci. USA 84:204. 3. Calvo, J. et al. (1999) Tissue Antigens 54:128. 4. Brown, M.H. and E. Lacey (2010) J. Immunol. 185:6068. 5. Van de Velde, H. et al. (1991) Nature 351:662. 6. Vera, J. et al. (2009) Proc. Natl. Acad. Sci. 106:1506. 7. Azzam, H.S. et al. (1998) J. Exp. Med. 188: 2301. 8. Hawiger, D. et al. (2004) Immunity 20:695. 9. Tarakhovsky, A. et al. (1995) Science 269:535. 10. Friedlein, G. et al. (2007) J. Immunol. 178:6821. 11. Ordonez