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CD50 (CDw50, CDW50, ICAM-3, ICAM-R, Intercellular adhesion molecule 3)

CD50 (CDw50, CDW50, ICAM-3, ICAM-R, Intercellular adhesion molecule 3)

Cat no: C2405-05S

Supplier: United States Biological
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Human CD50 is an adhesion molecule that interacts with LFA-1 (CD18/CD11a) and provides a costimulatory signal for T cells (1, 2, 3) which is thought to be essential for initiation of immune responses. Recognizes the D1 domain of the ICAM-3 molecule of 130kD (4), and blocks binding of CD11a (LFA-1) to CD50 (ICAM-3). Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: Paraffin Flow Cytometry: 5x10e5 cultured human Jurkat cells per tube were washed and pre-incubated 5 min on ice with 20ul of 250ug/ml human IgG (to block non specific binding) after which they were incubated 45 min on ice with 80ul of anti-CD50 antibody at 10ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.84 log10 fluorescent units when compared to a Mouse IgG2b negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2405-05S
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry
Size: 100ug
Form: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride.
P type: Mab
Isotype: IgG2b
Purity: Purified by Protein A affinity chromatography.
References: 1. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford (1995) p. 1546-1547, 1578-1579, 2. C.L. Holness, et al, (1995) J Biol Chem 270:877-884, 3. Y. van Kooyk, et al, (1996) J Exp Med 183:1247-1252, 4. Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 403-409.
Additional info: Recognizes human CD50.

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