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CD50 (Intercellular Adhesion Molecule, ICAM-3)

Cat no: C2406-13A


Supplier: United States Biological
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CD50 is a single chain polypeptide with a MW of 120kD. The protein is heavily glycosylated and resistant to treatment with phosphatidylinositol phospholipase C, and therefore probably not PI-anchored to the membrane. The antibody was provisionally clustered as CDw50 in Vienna at the IV and as CD50 in Boston at the V international workshop on human leukocyte differentiation antigens. Applications: Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2406-13A
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunoprecipitation
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. Leukocyte Typing VI., (1997), Kishimoto, T., et al., (Eds.), Garland Publishing Inc.
Additional info: Recognizes an epitope in the D2 domain of human CD50 (ICAM-3) at ~120-130kD.

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