CD50 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. CD50 is constitutively and abundantly expressed by all leukocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signaling molecule (1).
Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:10-100
Western Blot: 1:1000-10,000
Immunohistochemistry (Paraffin): 1:500-1000
Immunocytochemistry: 1:100-250
Immunoprecipitation: 1:10-100
Optimal dilutions to be determined by the researcher.
Positive Control:
Western Blot: U937, Jurkat, HuT-78, Ramos, and THP-1 cell lysates
Immunohistochemistry: Human spleen tissue, human colon tissue, human tonsil tissue
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
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