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CD51 (DKFZp686A08142, Integrin alpha-V, MSK8, Vitronectin receptor subunit alpha, VNRA)

CD51 (DKFZp686A08142, Integrin alpha-V, MSK8, Vitronectin receptor subunit alpha, VNRA)

Cat no: C2405-12F

Supplier: United States Biological
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Human CD51 is an integrin alpha v chain which can associate with five different integrin beta chains to form various adhesion molecules. The alpha v beta 3 integrin complex binds vitronectin at the RGD sequence and also can bind to fibrinogen, von Willebrand factor, thrombospondin, fibronectin, osteopontin and collagen. Recognizes the alpha v molecule of 160kD. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 cultured UM-SCC (squamous cell carcinoma) per tube were harvested by trypsinization, washed and pre-incubated 5 min with 20ul of 250ug/ml human IgG (to block nonspecific binding), after which they were incubated 45 min on ice with 80ul of anti-CD51 antibody at 10ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.32 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2405-12F
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 100ug
Form: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. R.O. Hynes, (1992) Cell 69:11-25, 2. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford (1995) p. 1663-1664.
Additional info: Recognizes human CD51.

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