Home  >  Products  >  CD53 (Cell surface glycoprotein CD53, Leukocyte surface antigen CD53, MOX44, Tetraspanin-25, TSPAN25, Tspan-25) (FITC)
CD53 (Cell surface glycoprotein CD53, Leukocyte surface antigen CD53, MOX44, Tetraspanin-25, TSPAN25, Tspan-25) (FITC)

CD53 (Cell surface glycoprotein CD53, Leukocyte surface antigen CD53, MOX44, Tetraspanin-25, TSPAN25, Tspan-25) (FITC)

Cat no: C2408-11G

Supplier: United States Biological
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Human CD53 is a tetraspan cell surface glycoprotein found on all hemopoetic cells except platelets and red blood cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 cultured human Jurkat cells per tube were incubated 45 min on ice with 80ul of anti-CD53 antibody at 10ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.37 log10 fluorescent units when compared to a Mouse IgG2b negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Catalogue number: C2408-11G
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 100ug
Form: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride. Labeled with fluorescein isothiocyanate (FITC).
P type: Mab
Isotype: IgG2b
Purity: Purified by Protein A affinity chromatography.
References: 1. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 534, 541, 2. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford (1995) p. 556-559, 3. Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 517-519.
Additional info: Recognizes human CD53.

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