Home  >  Products  >  CD54 (BB2, Cell Surface Glycoprotein P3.58, Human Rhinovirus Receptor, Intercellular Adhesion Molecule 1, ICAM 1, ICAM-1, Major Group Rhinovirus Receptor, MALA-2, MyD10, P3.58) (Low Endotoxin)
CD54 (BB2, Cell Surface Glycoprotein P3.58, Human Rhinovirus Receptor, Intercellular Adhesion Molecule 1, ICAM 1, ICAM-1, Major Group Rhinovirus Receptor, MALA-2, MyD10, P3.58) (Low Endotoxin)

CD54 (BB2, Cell Surface Glycoprotein P3.58, Human Rhinovirus Receptor, Intercellular Adhesion Molecule 1, ICAM 1, ICAM-1, Major Group Rhinovirus Receptor, MALA-2, MyD10, P3.58) (Low Endotoxin)

Cat no: C2409-11C

Supplier: United States Biological
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CD54 (ICAM-1) permits antigen-independent adhesion between lymphocytes and their targets and may be used to study cell-cell interactionsor for rhinovirus and malarial studies. The LFA-1 binding site is located in domain 1 of ICAM-1. CD54 is a membrane glycoprotein with a wide tissue distribution that includes vascular endothelium and many cells of the immune system. CD54 is weakly expressed on resting peripheral blood lymphocytes. Upon activation by mitogens, the CD54 antigen is strongly expressed on B-cells, T-cells, macrophages and granulocytes. Applications: Suitable for use in Flow Cytometry, Functional Assays, Immunohistochemistry, Immunoprecipitation, Western Blot and Immunofluorescence. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:25-1:50; 10ul labels 10e6 cells in 100ul Immunohistochemistry (frozen/paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Optimal dilutions to be determined by the researcher. Hybridoma: PAI myeloma cells with spleen cells from Balb/c mice. Recommended Secondary Antibodies: I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo Recommended Negative Control: I1904-82G2: IgG1, Murine Negative Control (For rat tissue) (Low Endotoxin) Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2409-11C
Reactivities: Rat
Hosts: Mouse
Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 500ug
Form: Supplied as a liquid in PBS, pH 7.4. No preservatives added.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography. Endotoxin: <0.01EU/ug.
References: 1. Tamatani, T and Miyasaka, M. (1990) Int. Immunol. 2: 165-171, 2. Tamatani, T. et al. (1991) Eur. J. Immunol. 21: 855-858, 3. Etienne, S. et al. (1998) J. Immunol. 161: 5755-5761, 4. Etienne, S. et al. (2000) J. Immunol. 165: 3375-3383, 5. McKechnie, N. M. et al. (2002) Invest Ophthalmol Vis Sci. 43:411-8.
Additional info: Recognizes rat CD54. Functionally inhibits homotypic aggregation of PHA blasts

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