CD54 is a 90kD type 1 transmembrane protein which is also known as ICAM-1. CD54 is broadly expressed on haematopoietic and endothelial cells. CD54 is also expressed by many cells following activation by inflammatory mediators. The CD54 molecule is a ligand for integrins CD11a/CD18 and CD11a/CD18, and for fibrinogen. The molecule also acts as a receptor for rhinoviruses and erthyrocytes infected with Plasmodium falciparum.
Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:25-1:100; 10ul labels 10e6 cells in 100ul
Immunohistochemistry: frozen sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.