The adhesion molecule ICAM 1 belongs to the immunoglobulin superfamily, C2 subset; it is a ligand for the Integrins LFA 1 and MAC 1 and for CD43.
Applications:
Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Immunofluorescence: 1:10
Immunohistochemistry (Frozen): 1:10 Fixation of tissue sections in acetone for 10 min is recommended, followed by a 10 minute incubation in chloroform. Incubation with a pretested dilution of the antibody is advised to be followed by a biotin conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin.
Immunocytochemistry: 1:10 In vitro cultured cells can be fixed with 1% paraformaldehyde and kept in PBS plus azide before staining
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.