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CD54 (D1) (Intercellular Adhesion Molecule 1, ICAM-1, Major Group Rhinovirus Receptor, ICAM1)

CD54 (D1) (Intercellular Adhesion Molecule 1, ICAM-1, Major Group Rhinovirus Receptor, ICAM1)

Cat no: C2409-11M

Supplier: United States Biological
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Human CD54 (ICAM-1) mediates cell adhesion by binding to the integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). CD54 expression on resting peripheral blood leukocytes is weak but is upregulated on activated T and B lymphocytes and on monocytes. Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: Capture Immunohistochemistry: Fixed cells and tissue Flow Cytometry: 1ug/ml Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x10e5 cultured Raji cells were incubated 45 min on ice with 80ul of C2409-11M at 1ug/ml. 2. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. 3.Cells stained positive with a mean shift of 1.58 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2409-11M
Reactivities: Human, Porcine
Hosts: Mouse
Applications: ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride.
P type: Mab
Isotype: IgG1,k
Purity: Purified by Protein A affinity chromatography.
References: 1. A.R. Berendt, et al, (1992) Cell 68:71-81, 2. I. Dransfield, et al, (1992) J Cell Biol 116:1527-1535, 3. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1548-1550, 4. P.L. Reilly, et al, (1995) J Immunol 155:529-532.
Additional info: Recognizes the D1 domain of human CD54 at~90kD. Inhibits CD54 binding to LFA-1. Species crossreactivity: porcine

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