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CD54 (D2) (Intercellular Adhesion Molecule 1, ICAM-1, Major Group Rhinovirus Receptor, ICAM1)

CD54 (D2) (Intercellular Adhesion Molecule 1, ICAM-1, Major Group Rhinovirus Receptor, ICAM1)

Cat no: C2409-11L

Supplier: United States Biological
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Human CD54 (ICAM-1) mediates cell adhesion by binding to the integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). CD54 expression on resting peripheral blood leukocytes is weak but is upregulated on activated T and B lymphocytes and monocytes. Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilution: ELISA: Detection Flow Cytometry: 5ug/ml (80ul) Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x10e5 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80ul of C2409-11L at 5ug/ml. 2. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.94 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2409-11L
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Flow Cytometry
Size: 100ug
Form: Supplied as a liquid in PBS. Preservative free
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. A.R. Berendt, et al, (1992) Cell 68:71-81, 2. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1548-1550, 3. P.L. Reilly, et al, (1995) J Immunol 155:529-532.
Additional info: Recognizes the D2 domain of the human CD54 molecule of ~90kD. Inhibits CD54 binding to LFA-1.

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