Antigen-specific cell contacts in the immune system are strengthened by antigen-nonspecific interactions, mediated in part by lymphocyte-function associated (LFA) antigens. ICAM-1 (intercellular adhesion molecule-1) has been defined as a ligand for LFA-1. Monoclonal antibodies to ICAM-1 block T lymphocyte adhesion to fibroblasts and endothelial cells; they disrupt the interaction between cytotoxic T cells and target cells (1). The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. However, ICAM-1 is also used as a receptor by the major group of human rhinoviruses and is a catalyst for the subsequent viral uncoating during cell entry (2). ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells; its expression is regulated by inflammatory cytokines (1).
Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:100-500
Western Blot: 1:1000-10,000
Immunohistochemistry (Paraffin): 1:100 -250
Immunoprecipitation: 1:10 -100
Optimal dilutions to be determined by the researcher.
Positive Control:
Western Blot: Raji, Ramos, A549 treated with TNF-alpha, A549, HUVEC treated with TNF-alpha cell lysates.
Immunohistochemistry: Human kidney tissue, human tonsil tissue
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
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