ICAMs are members of the Ig superfamily of calcium-independent transmembrane glycoproteins. ICAM-1 is a ligand for lymphocyte function-associated (LFA) and Mac-1 integrins and the major human rhinovirus receptor. The primary function of ICAM-1 is to provide adhesion between endothelial cells and leukocytes after stress or injury. The human ICAM-1 gene codes for a 505aa transmembrane glycoprotein containing a 29aa cytoplasmic domain, a 23aa transmembrane domain, and a 453aa extracellular domain. Recombinant human ICAM-1 is a 49.5kD glycoprotein comprising the extracellular domain (453aa residues) of ICAM-1. Monomeric glycosylated ICAM-1 migrates at an apparent molecular weight of approximately 72.0-80kD by SDS-PAGE analysis under reducing conditions.
Source:
Recombinant protein corresponding to human ICAM-1, expressed in CHO cells.
Biological Activity:
ICAM-1 supported the adhesion of more than 50% PMA-stimulated HSB2 cells to plates coated at 25ng/ml ICAM-1 concentration.
Endotoxin:
<0.1ng/ug (1EU/ug)
AA Sequence:
QTSVSPSKV ILPRGGSVLV TCSTSCDQPK LLGIETPLPK KELLLPGNNR KVYELSNVQE DSQPMCYSNC PDGQSTAKTF LTVYWTPERV ELAPLPSWQP VGKNLTLRCQ VEGGAPRANL TVVLLRGEKE LKREPAVGEP AEVTTTVLVR RDHHGANFSC RTELDLRPQG LELFENTSAP YQLQTFVLPA TPPQLVSPRV LEVDTQGTVV CSLDGLFPVS EAQVHLALGD QRLNPTVTYG NDSFSAKASV SVTAEDEGTQ RLTCAVILGN QSQETLQTVT IYSFPAPNVI LTKPEVSEGT EVTVKCEAHP RAKVTLNGVP AQPLGPRAQL LLKATPEDNG RSFSCSATLE VAGQLIHKNQ TRELRVLYGP RLDERDCPGN WTWPENSQQT PMCQAWGNPL PELKCLKDGT FPLPIGESVT VTRDLEGTYL CRARSTQGEV TRKVTVNVLS PRYE
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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