CD54, Recombinant, Human (Intercellular Adhesion Molecule, ICAM-1)

ICAM-1 (CD54) is a single chain membrane glycoprotein expressed on the surface of a variety of non-haematopoietic and haematopoietic cell types and has roles in signal transduction, cell signalling and lymphocyte adhesion. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory conditions. Intercellular adhesion molecule 1 (ICAM-1) is the cellular receptor for the major group of human rhinovirus serotypes, including human rhinovirus 14 (HRV14) and HRV16.\n\nRecombinant Human ICAM1 is expressed in E. coli containing 455 aa28-483 fused to an amino terminal hexahistidine tag.Purified by proprietary chromatographic techniques.\n\nApplications: \nSuitable to be used directly as a positive control in Western Blot, ELISA and immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions: Optimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2409-16

Size

2ug

Form

Supplied as a liquid in PBS, 2mM Imidazole, 50% glycerol.

Purity

(same/more than) 95% (SDS-PAGE). Single band on Western Blot.

References

1. ICAM-1 Expression in Adipose Tissue: Effects of Diet-Induced Obesity in Mice. Am J Physiol Cell Physiol 2006 Jun 28. 2. Combination of systemic thioacetamide (TAA) injections and ethanol feeding accelerates hepatic fibrosis in C3H/He mice and is associated with intrahepatic up regulation of MMP-2, VEGF and ICAM-1. J Hepatol 2006 May 3. 3. COX-2 is associated with cadmium-induced ICAM-1 expression in cerebrovascular endothelial cells.Toxicol Lett 2006 May 9. 4. Plasma protein binding of an antisense oligonucleotide targeting human ICAM-1 (ISIS 2302). Oligonucleotides 2006 Summer;16(2):169-80. 5. Effects of mechanical tensile stress on the expression of ICAM-1 mRNA in osteoblasts differentiated from rBMSCs; Sichuan Da Xue Xue Bao Yi Xue Ban 2006 May;37(3):438-41