CD55 (Decay-accelarating factor/DAF) is a glycoprotein member of the RCA family of molecules. It is found on blood cells, epithelium and endothelium, and serves both as a receptor for CD97, and a negative regulator of the C3 convertases, C4b2a and C3bBb. Mature mouse CD55 is the product of two genes that arose by duplication. There is a 55-60kD, 356aa, GPI-linked form that is ubiquitously expressed. This molecule contains four SUSHI domains (aa35-285), a Ser/Thr-rich region aa288-362, and a GPI-anchor at Gly362. There is also a 50kD, 379aa, type I transmembrane form that is testis-associated. It shows the same domain architecture and is 93% aa identical to the GPI-form. At least four GPI gene isoforms exist. They diverge after Ile285 and show deletions and substitutions. Over (aa35-359), mouse CD55 is 66% and 50% aa identical to rat and human CD55, respectively.
Applications:
Suitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 25ug/ml; Add 10ul of diluted solution to 1-2.5x10e5 cells in a total reaction volume (same/less than) 200ul.
Direct ELISA: 0.5-1ug/ml; The detection limit for recombinant mouse CD55 is ~2ng/well.
Western Blot: 2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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