Neural cell adhesion molecule (NCAM) is a glycoprotein with 5 extracellular immunoglobulin-like domains followed by two fibronectin type III repeats. NCAM mediates cell-cell adhesion in neural cells as well as a variety of other cell types. NCAM is expressed widely during embryonic development but is restricted in the adult brain. Many isoforms are produced from the alternate splicing of a single gene with 26 exons. This antibody reacts with higher molecular weight human isoforms; NCAM 140 and NCAM 180.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 4.0ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.