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CD56 (MSK39, NCAM, NCAM-1, N-CAM-1, Neural cell adhesion molecule 1)

CD56 (MSK39, NCAM, NCAM-1, N-CAM-1, Neural cell adhesion molecule 1)

Cat no: C2254-68B

Supplier: United States Biological
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Human CD56 is an adhesion molecule from the Ig superfamily which is restricted to NK cells in the immune system. It is believed that NK cells form a first line of defense against tumor cells and cells infected with bacteria and viruses. Applications: Suitable for use in Flow Cytometry, Western Blot, ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Acetone fixation Flow Cytometry: 80ul (10ug/ml) to label 5x10e5 human peripheral blood mononuclear cells Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 ficoll prepared human peripheral blood mononuclear cells were washed and pre-incubated for 5 minutes with 20ul of 250ug/ml human IgG (to block non-specific binding) after which they were incubated 45 minutes on ice with 80ul of C2254-68B at 10ug/ml. 2. Cells were washed twice and incubated 2 with IgG (FITC) goat anti-mouse, after which they were washed three times, fixed and analyzed by FACS. 3. A 15% sub population of the cells stained positive with a mean shift of 0.91 log10 fluorescent units when compared to a mouse IgG1 negative control at a similar concentration. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2254-68B
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride.
P type: Mab
Isotype: IgG1,k
Purity: Purified by Protein A affinity chromatography.
References: 1. S.P. Bourne, et al, (1991) J Neuro-Oncol 10:111-119, 2. T.L. Whiteside and R.B. Herberman, (1994) Clinical & Diagnostic Laboratory Immunology 1:125-133, 3. H. Spits, et al, (1995) Blood 85:2654-2670, 4. R. Gerardy-Schahn, M Eckhardt, (1994) Int J Cancer: Supp 8:38-42.
Additional info: Recognizes a stem region epitope of the human CD56 molecule.

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