CD57, Recombinant, Rat (CD57 Antigen, Beta-1,3-glucuronyltransferase 1, B3GAT1, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, Glucuronosyltransferase P, GLCATP, GlcAT-P, GlcUATP, GlcUAT-P, HNK1, HNK-1, LEU7, LEU7 Antigen, NK1, NK-1,

B3GAT1 is a key enzyme involved in human natural killer-1 (HNK-1) epitope synthesis. It adds a glucuronic residue to the terminal lactosamine residue (Galb1-4GlcNAc-) of a glycoprotein or glycolipid, which can be further sulfated to become the HNK-1 epitope, a unique trisaccharide structure, HSO3-3GlcAb1-3Galb1-4GlcNAc- (1, 2). The enzyme activity was found to be enhanced in the presence of sphingomyelin and phosphatidylinositol (3).The HNK-1 carbohydrate epitope is characteristically expressed on a series of cell adhesion molecules in addition to some glycolipids in the extracellular matrix and on the cell surface in the nervous system, where it is involved in cell-cell and cell-substratum interaction and recognition during the development of the nervous system (4). Like most known glycosyltransferases, B3GAT1 is a type II Golgi-resident transmembrane protein with a short N-terminal cytoplasmic domain and a single-pass transmembrane domain followed by an enzymatic domain in the lumen of Golgi apparatus. The enzyme activity was assayed using a phosphatase-coupled method (5).\n\nSource: \nRecombinant corresponding to aa75-347 from rat B3GAT1 fused with 6-His-tag at N-terminal (human CD33) expressed in mouse myeloma cell line, NSO.\n\nMolecular Weight: \n~32kD\n\nEndotoxin: (same/less than)1EU/1ug (LAL)\n\nBiological Activity:\nMeasured by its ability to transfer GlcA from UDP-GlcA to lactose. The specific activity is >725 pmol/min/ug, as measured under the described conditions.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2411-23R

Size

20ug

Form

Supplied as a liquid in Tris, sodium chloride, DTT.

Purity

~80% (SDS-PAGE)

References

1. Terayama, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6093. 2. Shogo, O. et al. (1992) J. Biol. Chem. 267: 22711. 3. Kakuda, S. et al. (2005) Glycobiology 2:203. 4. Bollensen, E. and Schachner, M. (1987) Neurosci Lett. 82:77. 5. Wu, Z.L. et al. (2001) Glycobiology doi:10.1093/glycob/cwq204.