Home  >  Products  >  CD58 (Ag3, FLJ23181, FLJ43722, LFA3, LFA-3, Lymphocyte function-associated antigen 3, Surface glycoprotein LFA-3) (Biotin)
CD58 (Ag3, FLJ23181, FLJ43722, LFA3, LFA-3, Lymphocyte function-associated antigen 3, Surface glycoprotein LFA-3) (Biotin)

CD58 (Ag3, FLJ23181, FLJ43722, LFA3, LFA-3, Lymphocyte function-associated antigen 3, Surface glycoprotein LFA-3) (Biotin)

Cat no: C2412-05L

Supplier: United States Biological
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Human CD58 is a cell surface glycoprotein that functions as an adhesion molocule and mediates costimulatory activity through its ligand CD2, which is expressed on T and natural killer cells (1,2). CD58 has wide cellular distribution on both hemopoietic and non-hemopoietic cells with strong expression on macrophages and memory T cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 cultured human Nalm-6 cells were washed and incubated 45 min on ice with 80ul of anti-CD58/Biotin at 10ug/ml. Cells were washed twice and incubated with Streptavidin/R-PE after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.1 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cells were pre-incubated 10 min with 20ul of 0.5mg/ml anti-CD58 antibody. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2412-05L
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Conjugates: Biotin
Size: 100ug
Form: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 500mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 15% glycerol. Labeled with biotin.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 414-415, 2. A.R. Arulanandam, et al, (1994) J Exp Med 180:1861-1871.
Additional info: Recognizes the CD58 glycoprotein of about 60-70kD. Inhibits HLA-DR mediated T cell cytotoxicity.

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