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CD60

CD60

Cat no: C2413-75B

Supplier: United States Biological
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Human CD60 antigen is a glycolipid determinant found on about 30% of peripheral blood T cells. The glycolipid determinant is linked to a glycoprotein which can transduce activation signals. Applications: Suitable for use in Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: Fixed cells or tissues. Flow Cytometry: 5x10e5 cultured Molt-4 human tumor cells were washed and incubated 45 min on ice with 80ul of anti-CD60 at 5ug/ml. Cells were washed twice IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.3 log10 fluorescent units when compared to a Mouse IgM negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2413-75B
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride.
P type: Mab
Isotype: IgM
Purity: Purified by Protein A affinity chromatography.
References: 1. J.B. Higgs, et al, (1988) J Immunol 140:3758-3765, 2. D.A. Fox, et al, (1990) Cell Immunol 128:480-489, 3. B. Kniep, et al, (1993) Blood 82:1776-1786, 4. E.P. Rieber & G. Rank, (1994) J Exp Med 179:1385-1390, 5. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 398-404, 7. Mason, D., et al. 2002. Leucocyte typing VII. Oxford University Press, New York
Additional info: Recognizes a 9-0-acetylated disialosyl group linked to human CD60. Activates T cells.

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