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CD61 (Glycoprotein IIIa, Integrin beta3)

CD61 (Glycoprotein IIIa, Integrin beta3)

Cat no: C2405-18C

Supplier: United States Biological
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CD61 associates with either the alpha IIb integrin (CD41) or the alpha V integrin (CD51) at the cell surface. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, platelets and osteoclasts in association with CD51. CD61 is a receptor for fibrinogen, fibronectin, vWF, vitronectin and thrombospondin. Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:00 -1:200 Use 10ul of the suggested working dilution to label 1x10e6 cells in 100ul. Requires protein digestion pre-treatment of paraffin sections e.g. trypsin prior to staining. Western Blot: Detects a band/s of approximately 90kD in platelet lysates Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from immunized Balb/c mice were fused with cells of the NS1 myeloma cell line. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2405-18C
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Western Blot
Size: 200ug
Form: Supplied as a liquid in PBS, pH7.4, 0.09% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
References: 1. Phillips, D.R. et al. (1991) Cell 65:359-362. 2. Hynes, R.O. (1992) Cell 69:11-25. 3. Michelson, A. D. et al. (1995) Leucocyte Typing V. Oxford University Press p 1230-1231. 4. Gatter, K. et al. (1998) Histopathology 13:257-267.
Additional info: Recognizes human CD61, a 105kD glycoprotein, which is also known as integrin beta 3 chain.

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