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CD61 (Integrin beta-3, Platelet Membrane Glycoprotein IIIa, GPIIIa, ITGB3, GP3A) (FITC)

CD61 (Integrin beta-3, Platelet Membrane Glycoprotein IIIa, GPIIIa, ITGB3, GP3A) (FITC)

Cat no: C2414-21T

Supplier: United States Biological
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CD61, a 105kD glycoprotein, is also known as integrin beta 3 chain. This molecule associates with either the alpha IIb integrin (CD41) or the alpha V integrin (CD51) at the cell surface. CD61 is expressed on platelets and megakaryocytes in association with CD41 and on endothelial cells, monocytes, platelets and osteoclasts in association with CD51. CD61 is a receptor for fibrinogen, fibronectin, vWF, vitronectin and thrombospondin. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 1x10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2414-21T
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 100 tests
Form: Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile dH2O. Labeled with fluorescein isothiocyanate (FITC).
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
References: 1. Phillips, D.R., et al., (1991), "GPIIb-IIIa: the responsive integrin", Cell, 65: 359-362. 2. Hynes, R.O., (1992), "Integrins: versatility, modulation, and signaling in cell adhesion", Cell, 69: 11-25. 3. Michelson, A.D., et al., (1995), "A panel of platelet MAb for the study of haemostasis and thrombosis in baboons", Leucocyte Typing V, Oxford University Press, p. 1230-1231. 4. Gatter, K., et al., (1998), "The immunohistological detection of platelets, megakaryocytes and thrombi in routinely processed specimens", Histopathology, 13: 257-267.
Additional info: Recognizes human CD61.

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