The gp IIb (CD41) antigen and the gp IIIa (CD61) antigen form the cell adhesion complex (gp IIb/IIIa) which is the receptor on platelets for fibrinogen, von Willebrand factor, fibronectin and vitronectin. The CD61 antigen is present on virtually all resting and activated platelets. endothelial cells, megakaryocytes and some leukemias of myeloid, erythroid, lymphoid origin.
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1ug labels 1x10e6 cells
Optimal dilutions to be determined by the researcher.
Hybridoma:
Myeloma cells with spleen cells from Balb/c mice.
Absorption: 492nm
Emission: 518nm
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.