Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:10
Immunohistochemistry (Frozen): 1:10; A small fresh skin biopsy can be incubated for 4 hours with TNF-alpha (1ng/ml), IL-1 (100U/ml) or LPS (1ug/ml) in tissue culture medium at 37 degrees C.
Optimal dilutions to be determined by the researcher.
IHC Positive Control:
Chimpanzee, rhesus monkey, cynomolgous monkey and baboon tissues.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.