Home  >  Products  >  CD62E (CD62 antigen-like family member E, CD62E, ELAM, ELAM1, ELAM-1, Endothelial leukocyte adhesion molecule 1, ESEL, E-selectin, LECAM2, Leukocyte-endothelial cell adhesion molecule 2)
CD62E (CD62 antigen-like family member E, CD62E, ELAM, ELAM1, ELAM-1, Endothelial leukocyte adhesion molecule 1, ESEL, E-selectin, LECAM2, Leukocyte-endothelial cell adhesion molecule 2)

CD62E (CD62 antigen-like family member E, CD62E, ELAM, ELAM1, ELAM-1, Endothelial leukocyte adhesion molecule 1, ESEL, E-selectin, LECAM2, Leukocyte-endothelial cell adhesion molecule 2)

Cat no: C2414-91L

Supplier: United States Biological
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Human CD62E (E-selectin) is an adhesion molecule expressed on activated endothelium and facilitates recruitment of leukocytes to sights of acute inflammation and immunological events. CD62E has affinity to LewisX (1). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Cultured human umbilical cord vein endothelial cells (HUVEC) were stimulated 4 hours with 10ng/ml Phorbol 12-Myristate 13-Acetate. Five x 105 cells per tube were harvested, washed and incubated 45 min on ice with 80ul of anti-CD62E antibody at 5ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.61 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2414-91L
Hosts: Mouse
Applications: Flow Cytometry
Size: 100ug
Form: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. T.P. Kogan, et al, (1995) J Biol Chem 270:14047-14055, 2. L. Tu, et al, (1996) J Immunol 157:3995-4004, 3. Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 416-419.
Additional info: Recognizes the lectin domain of human CD62E (2). Blocks the function of CD62E.

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