The CD62E antigen (97 to 115 kD), also known as E-selectin or endothelial leukocyte adhesion molecule-1 (ELAM-1), is expressed at sites of inflamm-ation. CD62E antigen is thought to trigger the initial tethering and rolling of leukocytes on endothelial surfaces, a prerequisite for leukocyte extravasation from blood into tissues. CD62E antigen expression is reported to be upregulated on vascular endothelium at sites of inflammation in a variety of disease states where neutrophil infiltration is evident.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry: Paraffin
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.