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CD62p (LECAM, P-Selectin, Endothelial Leukocyte Adhesion Molecule)

CD62p (LECAM, P-Selectin, Endothelial Leukocyte Adhesion Molecule)

Cat no: C2415-55P

Supplier: United States Biological
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CD62P is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leukocytes and endothelial cells. Applications: Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat-1:50. Use 10ul to label 10e6 cells in 100ul. Optimal dilution to be determined by researcher. Hybridoma: Spleen cells from immunized CD62P knock-out mice (strain C57/B6) were fused with cells of the NS-1 myeloma cell line. Recommended Negative Control: I1904-78R: IgG1 Negative Control Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2415-55P
Reactivities: Human, Porcine
Hosts: Mouse
Applications: Flow Cytometry, Immunoprecipitation
Size: 200ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
References: 1. Massaguer, A. et al. (2000) Tissue antigens 56:117-128. 2. Massaguer, A. et al. (2003) Immunopathol. 96:169-181.
Additional info: Recognizes the human CD62P cell surface antigen, a 140kD glycoprotein also known as P-selectin. Species Crossreactivity: porcine.

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