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CD63 (CD63 Antigen, Cd63 Molecule, Mast Cell Antigen AD1, MGC72893)

CD63 (CD63 Antigen, Cd63 Molecule, Mast Cell Antigen AD1, MGC72893)

Cat no: C2418-02L

Supplier: United States Biological
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CD63, is an intracellular type-III lysosomal glycoprotein and member of the tetraspanin (TM4SF) family, which is translocated to the cell surface upon activation of platelets, and therefore a potential indicator of platelet activation. Rat CD63 is expressed on mast cells, and studies using clone AD1, have shown that it is located near to FcER1, and that clone AD1 partially inhibits IgE-mediated histamine release. Applications: Suitable for use in Flow cytometry, Immunoprecipitation, Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: paraffin and frozen sections Flow cytometry: 1:10-1:200 Use 10ul to label 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2418-02L
Reactivities: Rat
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 25ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatograpy.
References: 1. Kitani, S. et al. (1991) A cell surface glycoprotein of rat basophilic leukemia cells close to the high affinity IgE receptor (Fc epsilon RI). Similarity to human melanoma differentiation antigen ME491 J. Biol. Chem. 266: 1903-1909.2. Boros, M. et al.. (1995) Response of mucosal mast cells to intestinal ischemia-reperfusion injury in the rat. Shock. 3: 125-131.
Additional info: Recognizes rat CD63.

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