Home  >  Products  >  CD63 (CD63 antigen, Granulophysin, LAMP-3, Lysosomal-associated membrane protein 3, ME491, Melanoma-associated antigen ME491, MLA1, Ocular melanoma-associated antigen, OMA81H, Tetraspanin-30, TSPAN30, Tspan-30)
CD63 (CD63 antigen, Granulophysin, LAMP-3, Lysosomal-associated membrane protein 3, ME491, Melanoma-associated antigen ME491, MLA1, Ocular melanoma-associated antigen, OMA81H, Tetraspanin-30, TSPAN30, Tspan-30)

CD63 (CD63 antigen, Granulophysin, LAMP-3, Lysosomal-associated membrane protein 3, ME491, Melanoma-associated antigen ME491, MLA1, Ocular melanoma-associated antigen, OMA81H, Tetraspanin-30, TSPAN30, Tspan-30)

Cat no: C2418-02N

Supplier: United States Biological
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Human CD63 is expressed on activated platelets and also on monocytes and macrophages. CD63 contains four hydrophobic transmembrane regions and is a member of the tetraspan family. CD63 appears to be involved with cell adhesion and associates with VLA-3 and VLA-6 integrins. Applications: Suitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 cultured HPB-MLT cells were washed and incubated 45 min on ice with 80ul of anti-CD63 antibody at 5ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.48 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2418-02N
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunocytochemistry
Size: 100ug
Form: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. F. Berditchevski, et al, (1995) J Biol Chem 270:17784-17790, 2. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1352-1364, 3. Sordat I, Sordat B, et al. (2002) Lab Investigations 82:1715-1724
Additional info: Recognizes human CD63.

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