CD63 (Melanoma)

CD63 is a lysosomal granule-membrane glycoprotein and has been detected on the surface and in the cytoplasma of a variety of cells, particularly in platelets; surface expression indicates lysosomal secretion. CD63, present only in azurophil granules of non-stimulated neutrophils, is strongly expressed at the surface of neutrophils after activation showing that CD63 is a good marker for granule release; this phenomenon has been also reported for basophils. CD63 is present in cell line PHA blasts, K562, U937, KG1a, RAJI. Both surface and cytoplasmic reactions are reported.\n\nApplications: Immunohistochemistry (formalin/paraffin only). Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.\n\nRecommended Dilution: 0.2-0.4ug/ml \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2418-05

Size

500ul

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide. Also available without BSA and azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Vennegoor C et al. Cancer Immunol. Immunother. 1986, 23:93\n2. Hagen, E.C., et al. 1986, Histopathology 10, 689.\n3. Palmer, A. A., et al., 1985, Pathology 17, 335.

Additional Info

Recognizes a heterogeneous 25-110kD human glycoprotein that is located mainly in the innerside of membranes of cytoplasmic vesicles in melanoma cells.