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CD63 Molecule (CD63) ELISA Kit

Cat no: ABIN424768


Supplier: Antibodies-online
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CD63 Molecule (CD63) ELISA Kit: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tetraspanin 30 (TSPAN30). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tetraspanin 30 (TSPAN30). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tetraspanin 30 (TSPAN30), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm
Catalogue number: ABIN424768
Size: 96 tests
Accession: P41731
Gene: 12512
Antigen: TSPAN30
Sensitivity: The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D. Value of 20 replicates of the zero standard added by their three standard deviations.
Assay range: 0.156-10 ng/mL
Sample type: Tissue Homogenate,Cell Lysate,Biological Fluids
Entrez gene: 12512

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