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CD65s (CDw65)

CD65s (CDw65)

Cat no: C2405-21D

Supplier: United States Biological
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Applications: Suitable for use in Flow Cytometry, Immunofluorescence, and Immunohistochemistry (frozen). Other applications not tested. Recommended Dilution: Flow Cytometry: Neat-1:200; 10ul labels 10e6 cells in 100ul Immunohistochemistry (frozen): 1:20 Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2405-21D
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry
Size: 200ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
P type: Mab
Isotype: IgM
Purity: Purified by ammonium sulfate precipitation.
References: 1. Gooi, H. C. et al. (1985) Differences in the fine specificities of monoclonal (Class A) antibodies to human myeloid cells. Clin. Exp. Immunol. 60: 151-158, 2. Lund-Johansen, F. et al. (1992) Activation of human phagocytes through carbohydrate antigens (CD15, SIALYL-CD15, CDw17 and CDw65). J. Immunol. 148: 3221-3229, 3. Knapp, W. et al. (1994) Flow cytometric analysis of cell-surface and intracellular antigens in leukemia diagnosis. Cytometry. 18: 187-198, 4. Lund-Johansen, F. et al. (1990) Flow cytometric assay for the measurement of human bone marrow phenotype, function and cell cycle. Cytometry. 11: 610-616.
Additional info: Recognizes human CD65s.

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