CD65, known as CD65s (VIM-2 antigen), a leukocyte carbohydrate antigen expressed by granulocytes, monocytes and leukaemic cells of myelomonocytic lineage. CD65s is aberrantly expressed on some acute myeloid leukemias (AML) and clone VIM-2 has been reliably used as a marker for distinguishing between mature and undifferentiated AML. During normal myelopoiesis, expression of CD65s follows the disappearance of the progenitor antigen CD34.
Cross-linking of the CD65s antigen using clone VIM-2, has been shown to induce phagocyte cytoplasmic calcium flux, oxidative burst and degranulation (2).
Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence and Functional Assays. Other applications not tested.
Recommended Dilution:
Flow Cytometry: (neat)1:200. Use 10ul of the suggested working dilution to label 1x10e6 cells in 100ul.
Immunohistochemistry (frozen): 1:20
Optimal dilution to be determined by researcher.
Endotoxin Level: <0.1EU/ug
Positive Control:
Human tonsil
Hybridoma:
Spleen cells from immunized Balb/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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