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CD66e (Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, CEA, DKFZp781M2392, Meconium antigen 100)

CD66e (Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, CEA, DKFZp781M2392, Meconium antigen 100)

Cat no: C1299-85C

Supplier: United States Biological
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Human CD66e is a heavily glycosylated GPI anchored protein capable of heterophilic and homophilic adhesion and may play a role in metastasis of cancer cells. Soluble CD66e is also known as carcino embryonic antigen(CEA). Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 cultured UM-SCC cells (squamous cell carcinoma) per tube were washed and pre-incubated 5 min with 20ul of 250ug/ml human IgG (to block non specific binding) after which they were incubated 45 min on ice with 80ul of anti-CD66e antibody at 10ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.33 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C1299-85C
Reactivities: Human
Hosts: Mouse
Applications: ELISA
Size: 100ug
Form: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 992-1015, 2. A. Gangopadhyay, et al, (1996) Cancer Res 56:4805-4810.
Additional info: Recognizes cell surface bound CD66e on human epithelial cells.

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