CD68 (DKFZp686M18236, GP110, Macrophage Antigen CD68 (Microsialin), Macrosialin, Scavenger Receptor Class D Member 1, SCARD1)

CD68, also called Scavenger Receptor D1 (SR-D1), is a type I transmembrane glycoprotein. Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablization. CD68 is a 110kD transmembrane (Type I) glycoprotein consisting of a polypeptide of 37408D (354 amino acids) predicted from the cDNA sequence that is highly expressed by human monocytes and tissue macrophages. CD68 plays a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes, lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, RIA and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul. Membrane permeabilization is required. Immunohistochemistry (Paraffin): 1:100. Requires protein digestion pre-treatment of paraffin sections (e.g. trypsin or pronase) Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from BLAB/c mice were fused with cells of the SP2/0-Ag-14 mouse myeloma cell line Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.