CD68 (DKFZp686M18236, GP110, Macrophage Antigen CD68 (Microsialin), Macrosialin, Scavenger Receptor Class D Member 1, SCARD1)

Cat no: C2423-05P

Supplier: United States Biological

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Description
Additional Information

Macrosialin is a heavily glycosylated transmembrane protein of 87-115kD, which is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablisation. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul Membrane permeabilization is required Immunohistochemistry: Frozen sections Immunohistochemistry (Paraffin): Requires antigen retrieval using heat treatment prior to staining paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. (See reference 13, Devey et al, Mol. Ther. 2009) Western Blot: Non-reducing conditions recommended Optimal dilutions to be determined by the researcher. Recommended Negative Control: I1904-82D1: IgG2a, Rat Negative Control Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	C2423-05P
Reactivities:	Mouse
Hosts:	Rat
Applications:	Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Size:	250ug
Form:	Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
P type:	Mab
Isotype:	IgG2a
Purity:	Purified by Protein G affinity chromatography.
Alternative names:	CD68 Antigen, CD68 Molecule, Monocytes/Macrophages
References:	1. Ramprasad, M.P. et al. (1996)CProc. Natl. Acad. Sci. 93: 14833-14838. 2. Rabinowitz, S.S. & Gordon, S. (1991) J. Exp. Med. 174: 827-836. 3. Da Silva, R.P & Gordon, S. (1999). Biochem. J. 338: 687-694. 4. Schleicher, U. et al. (2005) Blood 105: 1319-1328. 5. von Lukowicz, T. et al. (2008) Cardiovasc. Res. 78: 158-166 6. Kassim, S. et al. (2010) PloS ONE 5: e13424. 7. Rahaman, S.O. et al. (2011) J Biol Chem. Jan 5. [Epub ahead of print] 8. Frossard, J.L. et al. (2011) RJ Clin Pathol. Feb 23. [Epub ahead of print] 9. West, E.L. et al. (2010) Stem Cells. 28: 1997-2007. 10. Lopez, M.E. et al. (2011) J Neurosci. 31: 4367-78. 11. Jayagopal, A. et al. (2009) Nanotechnology. 20: 165102. 12. Leung, V.W. et al. (2009) Am J Pathol. 175: 1757-67. 13. Devey, L. et al. (2009) Mol Ther. 17: 65-72. 14. Lu, W. et al. (2010) Biomaterials. 31: 2617-26. 15. de Beer, M.C. et al. (2003) J Lipid Res. 44: 674-85. 16. Song, L. et al. (2011). J Lipid Res. 52: 1542-50. 17. Daldrup-Link, H.E. et al. (2011) Clin Cancer Res. Jul 26. [Epub ahead of print]
Additional info:	Recognizes mouse CD68. Detects surface macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.

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CD68 (DKFZp686M18236, GP110, Macrophage Antigen CD68 (Microsialin), Macrosialin, Scavenger Receptor Class D Member 1, SCARD1)

Cat no: C2423-05P

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