CD68, Recombinant, Human (MACROSIALIN, DKFZp686M18236, Macrophage antigen, SCARD1, Scavenger receptor class D member1)

CD68, also called Scavenger Receptor D1 (SR-D1), is a type I transmembrane glycoprotein. Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablization. \nCD68 is a 110kD transmembrane (Type I) glycoprotein consisting of a polypeptide of 37408D (354 amino acids) predicted from the cDNA sequence that is highly expressed by human monocytes and tissue macrophages. CD68 plays a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes, lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.\n\nRecombinant CD68 shows a band at 38kD on SDS-PAGE commassie blue stained gel.\n\nDimers and Aggregates:\n(same/less than) 1% as determined by coomassie blue SDS-PAGE gel analysis.\n\nEndotoxin:\n(same/less than) 0.1ng/ug (IEU/ug) of CD68.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2423-03A

Size

2ug

Form

Supplied as a liquid in 50mM Tris-Acetate, pH 7.5, 1mM EDTA, 20% glycerol.

Purity

(same/more than) 90% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Immunohistochemical localization of macrophage CD68+, HLA-DR+, L1+ and CD44+ subsets in uterine endometrium during different phases of menstrual cycle. Kar M, Sengupta J, Bhargava VL, Indian J Physiol Pharmacol 2004 Jul;48(3):293-303 2. CD68 is not a macrophage-specific antigen * Authors' reply. Beranek JT, Kunisch E, Kinne RW, Lungershausen W, Ann Rheum Dis 2005 Feb;64(2):342-4 3. Macrophage/histiocytic antigen CD68 expression in neoplastic and reactive lymph nodes. Mazur G, Halon A, Kuliczkowski K, Rocz Akad Med Bialymst 2004;49 Suppl 1:73-5 4. Origin of stellate giant cells in oral fibrous lesions determined by immunohistochemical expression of vimentin, HHF-35, CD68 and factor XIIIa. Souza LB, Andrade ES, Freitas RA, Pathology 2004 Aug;36(4):316-20 5. CD68 and MCP-1/CCR2 expression of initial biopsies reflect the outcomes of membranous nephropathy. Yoshimoto K, Wada T, Sakai N, Nephron Clin Pract 2004;98(1):c25-34 6. Granuloma annulare with a mycosis fungoides-like distribution and palisaded granulomas of CD68-positive histiocytes. Wu H, Barusevicius A, J Am Acad Dermatol 2004 Jul;51(1):39-44.

Alternative Names

DKFZp686M18236, Macrophage antigen CD68 (microsialin), GP110, SCARD1, Scavenger receptor class D member1