CD7

CD7 antigen is a cell surface glycoprotein of 40kD expressed on the surface of immature and mature T cells, and natural killer cells. It is the member of immunoglobulin gene superfamily and is the first T cell lineage associated antigen to appear in T cell ontogeny, being expressed in pre-thymic T cell precursors (preceding CD2 expression), and in myeloid precursors in fetal liver and bone marrow, and persisting in circulating T cells. While its precise function is not known, there is recent suggestion that the molecule functions as an Fc receptor for IgM. CD7 is the most consistently expressed T cell antigen in lymphoblastic lymphomas and leukemias, and is therefore a useful marker in the identification of such neoplastic proliferations. In mature post-thymic T cell neoplasms, it is the most common pan-T antigen to be aberrantly absent and its absence in a T cell population is a useful pointer to a neoplastic conversion. CD7 is immunoexpressed on 85% of mature peripheral T cells, the majority of post-thymic T cells, NK cells, some myeloid cells, T cell acute lymphoblastic leukemia/lymphoma, acute myelogenous leukemia and chronic myelogenous leukemia. Interestingly, CD7 is conspicuously absent in adult T cell leukemia/lymphoma and is not expressed in Sezary cells. Associated products: CD2, CD3, CD5, CD8, CD20, CD56, CD57, TdT. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (parraffin, frozen): 1:5-1:50 Optimal dilutions to be determined by the researcher. Positive Control: Tonsil, Lymph Node Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.