Human CD27 Ligand (also CD70) is a 30kD type 2 transmembrane glycoprotein that is a member of the TNF superfamily. It contains a 20aa cytoplasmic region and a 155aa extracellular domain that shows multiple b-strands. It is an inducible trimer that occurs on B cells, T cells and NK cells. It regulates T cell dependent B cell differentiation into plasma cells and likely to promotes clonal expansion of T cells. Human CD27 Ligand extracellular region is 64% aa identical to mouse and rat CD27 Ligand extracellular regions.
Applications:
Suitable for use in ELISA, Western Blot and Flow Cytometry. Other applications not tested.
Recommended Dilution:
Western Blot: 1ug/ml
Flow Cytometry: 2.5ug labels 10e6 Raji cells from human Burkitt