Home  >  Products  >  CD71 (CD71 Antigen, p90, T9, Transferrin Receptor, TFR, TR, Transferrin Receptor Protein 1, TFR 1, TFR1, TFR-1, TFRC, TRFR)
CD71 (CD71 Antigen, p90, T9, Transferrin Receptor, TFR, TR, Transferrin Receptor Protein 1, TFR 1, TFR1, TFR-1, TFRC, TRFR)

CD71 (CD71 Antigen, p90, T9, Transferrin Receptor, TFR, TR, Transferrin Receptor Protein 1, TFR 1, TFR1, TFR-1, TFRC, TRFR)

Cat no: C2426-02

Supplier: United States Biological
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
Read More on Supplier Website
Human CD71 is a type II transmembrane glycoprotein found on activated leukocytes. CD71 binds transferrin and mediates uptake of iron through endocytosis and recycling. Applications: Suitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5ug/ml Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x10e5 cultured HPB-MLT cells were washed and pre incubated 5 minutes with 20ul of 250ug/ml human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80ul of C2426-02 at 5ug/ml. 2. Cells were washed twice and incubated with IgG (FITC) Goat anti-Mouse, after which they were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 2.23 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2426-02
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunocytochemistry
Size: 100ug
Form: Supplied as a liquid in PBS. No preservative added.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
Alternative names: Transferrin Receptor (p90, CD71)
References: 1. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 455-460, 2. Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 524-527, 3. R Madrid, S Benichow, et al (2004) J Biol Chem 280: 5032-5044, 4. S Coleman, J C Guatelli, et al. (2006) J Virol 80(4): 1837-1849, 5. C C I Lee, A F Tarantal, et al. (2010) Exp Hematol 38(1): 46-54.
Additional info: Recognizes human CD71 at ~95kD.

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
Enquiry_down_arrow
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*
See Full Product Listings >
Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave